Type I & Type II Water
Type I Water
General Water is a premier provider of Type I water purification units, most commonly referred to as "systems." Type I water, also referred to as Clinical Laboratory Reagent Water (CLRW), deionized (DI), or ultra-pure, is water that meets the standards necessary for use in numerous type of critical laboratory applications.
Type I water is the grade required for critical laboratory applications such as HPLC mobile phase preparation, blanks and sample dilution in Gas Chromatography, HPLC, AA, ICP-MS and other advanced analytical techniques. It is necessary for the preparation of buffers and culture media for mammalian cell culture and IVF. Type I water is specified in the production of reagents for molecular biology applications (DNA sequencing, PCR) and is required in the preparation of solutions for electrophoresis and blotting.
Type I water is the grade required for critical laboratory applications such as HPLC mobile phase preparation, blanks and sample dilution in Gas Chromatography, HPLC, AA, ICP-MS and other advanced analytical techniques. It is necessary for the preparation of buffers and culture media for mammalian cell culture and IVF. Type I water is specified in the production of reagents for molecular biology applications (DNA sequencing, PCR) and is required in the preparation of solutions for electrophoresis and blotting.
Type II Water
Type II water is the grade used in general laboratory applications such as buffers, pH solutions, and microbiological culture media preparation and is required as the feed water to Type I water systems, clinical analyzers, cell culture incubators, and weatherometers. Type II water is also the grade designated for the preparation of reagents for chemical analysis or synthesis.
Using Type I grade water for Type II water applications is a common laboratory practice. Type I grade water decreases the risk of artifact generation during experimental procedures.
Using Type I grade water for Type II water applications is a common laboratory practice. Type I grade water decreases the risk of artifact generation during experimental procedures.
NCCLS Maximum Contaminant Levels in
Type I Purified Water
Contaminant
Parameter
Value for Type I Water
Ions
Resistivity at 25°C (Megohms/cm)
> 18.0
Conductivity at 25°C (microseimens/cm)
< 0.056
Organics
TOC (ppb)
< 10
Pyrogens
Eu/mL
< 0.03
Particulates
Size
<0.2 µm
Colloids
Silicia (ppb)
< 10
Bacteria
CFU/mL
< 1
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